Incorporation of 35SO4 into endogenous heparin by a microsomal fraction from mast cell tumors.
نویسنده
چکیده
A mouse mast cell tumor microsomal preparation which catalyzes the incorporation of 35S0., from 3’-phosphoadenosine 5’-phosphosulfate-33S into microsomal heparin has been described. This enzyme preparation is the same preparation that previously has been shown to catalyze the incorporation of sugars into a microsomal glycosaminoglycan related to heparin. It is suggested that polysaccharide polymerization and sulfation take place in close proximity in the cell.
منابع مشابه
Incorporation of “SO4 into Endogenous Heparin by a Microsomal Fraction from Mast Cell Tumors*
A mouse mast cell tumor microsomal preparation which catalyzes the incorporation of 35S0., from 3’-phosphoadenosine 5’-phosphosulfate-33S into microsomal heparin has been described. This enzyme preparation is the same preparation that previously has been shown to catalyze the incorporation of sugars into a microsomal glycosaminoglycan related to heparin. It is suggested that polysaccharide poly...
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1. Polysaccharide in a heparin-producing mouse mastocytoma was pulse-labelled in vivo with [35S] sulphate, and after various periods of time was isolated from subcellular fractions. Such fractions were recovered from tissue homogenates by consecutive centrifugations at 1000g for 10min, 20000g for 20min and 100000g for 1h. Initially the 35S-labelled polysaccharide formed occurred principally in ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 242 10 شماره
صفحات -
تاریخ انتشار 1967